FastQCFastQC Report
Sun 5 Jun 2022
EGAF00005308615

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005308615
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences102346505
Sequences flagged as poor quality0
Sequence length28
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTCGTTATCGGAATTAACCAGACAAA4764350.465511743659444No Hit
CCACCTACTAAGAACGGCCATGCACCAC1591170.15546891415588643No Hit
GTCTCGTTCGTTATCGGAATTAACCAGA1483280.14492727426305374No Hit
AAGCCGCAGGCTCCACTCCTGGTGGTGC1306740.12767802867328004No Hit
GCTGCGGATATGGGTACGGCCCGGCGCG1175320.11483733616502098No Hit
GATCGGAAGAGCACACGTCTGAACTCCA1044420.102047451449368Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CCACCAACTAAGAACGGCCATGCACCAC1025020.10015192995598628No Hit
TGCCCTCCAATGGATCCTCGTTAAAGGA1024120.10006399339186033No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)