FastQCFastQC Report
Sat 4 Jun 2022
EGAF00005308652

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005308652
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences74315114
Sequences flagged as poor quality0
Sequence length91
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT7248210.9753345732605618Clontech SMART CDS Primer II A (100% over 26bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4943000.6651406065258811No Hit
GCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT1562650.210273511791962Clontech SMART CDS Primer II A (100% over 24bp)
GTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTGCGCGC789810.1062785155654878No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)