FastQCFastQC Report
Tue 28 Jun 2022
EGAF00005308808

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005308808
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences52310061
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT13626092.604869835651692Clontech SMART CDS Primer II A (100% over 26bp)
GCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT3606220.6893931934049934Clontech SMART CDS Primer II A (100% over 24bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3020810.5774816435408094No Hit
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT1058590.20236833598798518Clontech SMART CDS Primer II A (100% over 25bp)
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT897990.1716667850951273Clontech SMART CDS Primer II A (100% over 21bp)
AGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT737060.14090214882372246Clontech SMART CDS Primer II A (100% over 22bp)
CAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT657630.125717689375281Clontech SMART CDS Primer II A (100% over 23bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)