FastQCFastQC Report
Thu 12 May 2022
EGAF00005308821

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005308821
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences108741162
Sequences flagged as poor quality0
Sequence length28
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTCGTTATCGGAATTAACCAGACAAA2471330.227267205402863No Hit
CCACCTACTAAGAACGGCCATGCACCAC1980570.18213618132938472No Hit
GTCTCGTTCGTTATCGGAATTAACCAGA1583540.14562470833261834No Hit
GCTGCGGATATGGGTACGGCCCGGCGCG1327240.1220549767529613No Hit
AAGCCGCAGGCTCCACTCCTGGTGGTGC1233550.1134391041361136No Hit
CCTTTAAGTTTCAGCTTTGCAACCATAC1174480.10800693853170339No Hit
GATCGGAAGAGCACACGTCTGAACTCCA1159000.10658337456427033Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)