FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005309357

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005309357
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences187095961
Sequences flagged as poor quality0
Sequence length100
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA244950.048.3926051
ATCGGAA306050.039.2170072
TCGGAAG336500.035.243773
CGGAAGA411450.028.7922134
TATGCCG206950.025.33383246-47
CTCGTAT237600.021.81859242-43
CGTATGC246500.021.333544-45
TCTCGTA243900.020.77063640-41
TGCCGTC269300.019.57314948-49
TCGTATG280800.019.3824742-43
ATGCCGT281000.019.03428546-47
GCCGTCT284800.018.7555548-49
ACACGTC325900.017.97674212-13
CGTCTGA322800.017.95841216-17
ATCTCGT309050.016.17144640-41
CATCTCG345850.015.20211438-39
ACGTCTG401850.014.73533214-15
CGTCTTC373150.014.70161150-51
CCGTCTT379400.014.1123550-51
CACGTCT453250.013.06429914-15
Produced by FastQC (version 0.11.5)