FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005309359

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005309359
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences201759362
Sequences flagged as poor quality0
Sequence length100
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA265750.048.5093571
ATCGGAA331200.038.953552
TCGGAAG365200.035.153833
CGGAAGA438200.029.182834
TATGCCG222600.025.28694546-47
CTCGTAT259400.021.67852642-43
CGTATGC263300.021.56401344-45
TCTCGTA263650.020.93583940-41
TGCCGTC282150.019.7825648-49
TCGTATG300500.019.43295942-43
ATGCCGT299650.018.996646-47
GCCGTCT299000.018.96640848-49
CGTCTGA351500.018.09442716-17
ACACGTC350750.018.0583812-13
ATCTCGT335700.016.51945740-41
CATCTCG372350.015.38166538-39
ACGTCTG437600.014.74502614-15
CGTCTTC390300.014.73399350-51
CCGTCTT401000.014.25879250-51
CACGTCT478800.013.535151514-15
Produced by FastQC (version 0.11.5)