FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005309361

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005309361
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences201772395
Sequences flagged as poor quality0
Sequence length100
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA272000.048.5984081
ATCGGAA335300.039.1147772
TCGGAAG370250.035.348123
CGGAAGA447050.029.0704084
TATGCCG223500.025.59866146-47
CTCGTAT260300.022.05929842-43
CGTATGC265300.021.83852644-45
TCTCGTA264900.021.33793840-41
TCGTATG300050.019.9359342-43
TGCCGTC289450.019.7003348-49
ATGCCGT299950.019.5522146-47
GCCGTCT305050.018.97796648-49
CGTCTGA354200.017.8900116-17
ACACGTC359450.017.84144212-13
ATCTCGT338650.016.60775840-41
CATCTCG375600.015.81909238-39
CGTCTTC395000.015.10189750-51
ACGTCTG431900.015.04868914-15
CCGTCTT408000.014.280827550-51
CACGTCT486200.013.26648614-15
Produced by FastQC (version 0.11.5)