FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005309365

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005309365
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences202298552
Sequences flagged as poor quality0
Sequence length100
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA273000.049.73851
ATCGGAA338000.040.135982
TCGGAAG365500.036.9192123
CGGAAGA449350.029.936974
TATGCCG233300.025.8425846-47
CGTATGC275650.022.21407544-45
CTCGTAT269650.022.13211842-43
TCTCGTA271400.021.43480740-41
TCGTATG309700.020.08210842-43
ATGCCGT306400.019.89960746-47
TGCCGTC303850.019.7583148-49
GCCGTCT316650.019.19716848-49
ACACGTC356850.018.76915712-13
CGTCTGA355950.018.60016316-17
ATCTCGT343500.017.05200840-41
CATCTCG378450.015.91902838-39
CGTCTTC403350.015.3684250-51
ACGTCTG435800.015.33666814-15
CCGTCTT409150.014.88052850-51
CACGTCT490400.013.83518614-15
Produced by FastQC (version 0.11.5)