FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005309367

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005309367
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences199430087
Sequences flagged as poor quality0
Sequence length100
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA266650.049.5991
ATCGGAA331550.040.067642
TCGGAAG365250.036.2230073
CGGAAGA431900.030.2442234
TATGCCG225650.026.15806446-47
CGTATGC268600.022.20359644-45
CTCGTAT263650.022.12043642-43
TCTCGTA267250.021.55012740-41
ATGCCGT303600.019.94519846-47
TGCCGTC299050.019.94392448-49
TCGTATG307950.019.84667842-43
GCCGTCT312750.019.34088948-49
ACACGTC347300.018.61949512-13
CGTCTGA350750.018.36833616-17
ATCTCGT333250.017.06355340-41
CATCTCG364100.016.17816438-39
CGTCTTC395550.015.49505950-51
ACGTCTG431800.015.17794214-15
CCGTCTT407700.014.84299250-51
CACGTCT483400.013.75709914-15
Produced by FastQC (version 0.11.5)