FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005309371

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005309371
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34599411
Sequences flagged as poor quality0
Sequence length76
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG73850.045.97162254
CGTCGAA76000.045.6820933
CGTATGC79050.043.08038355
ACGTCGA80850.042.94265732
CTCGTAT80650.041.92144853
TCTCGTA81650.038.7514352
TATGCCG95550.035.45843557
AGTCACG103900.033.9547428
GTCACGT107750.032.48146429
ATCTCGT99150.032.08838751
ACACGTC112900.031.09109513
TCACGTC113200.030.94856630
GTCGAAG118850.029.36061934
ACGTCTG127150.028.0192215
CACGTCT128500.027.64319414
ATGCCGT124750.027.49434958
CACGTCG130500.026.89940631
TCGAAGA141750.024.88870435
CCGTCTT143300.023.4713661
GCTTGAA153100.023.0683270
Produced by FastQC (version 0.11.5)