FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005309377

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005309377
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14533682
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG18450.028.26430554
CTCGTAT19100.027.11956653
CGTATGC20600.024.97485755
TCTCGTA19650.024.9354352
TATGCCG22450.023.0726157
TCCGGTA24800.021.87453835
ATCCGGT25750.021.33788734
ATCTCGT24500.020.5706451
ACACGTC28400.019.59310713
CCGGTAA10650.017.41747336
ATGCCGT32500.015.7221558
CACGTCT37500.014.93188714
ACGTCTG37300.014.91812715
GATCGGA39350.013.94081
CACACGT41100.013.53878812
CCGTCTT41350.013.37308161
ATCGGAA43300.012.8524532
CGCGAAA5801.8189894E-1212.0678846
TAGGTCG8300.011.8073043
GCACACG49900.011.64199811
Produced by FastQC (version 0.11.5)