FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005309379

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005309379
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30158154
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG133800.042.7124354
CTCGTAT132900.042.47572753
CGTATGC138450.041.30295655
TCTCGTA125450.041.2041152
TATGCCG143650.039.58935557
ATCTCGT138350.037.4633551
ATGCCGT163450.034.55805658
ACACGTC171600.033.99653213
ACGGAAC197800.032.768234
TCACACG182550.032.03552230
CCGTCTT177950.031.58470561
ACGTCTG187550.031.29192715
CACGTCT191300.030.733414
GCCGTCT190550.029.62474860
CACACGT199800.029.46095312
ACACGGA199550.029.39380832
TGCCGTC196550.029.0588159
CACACGG208500.028.19943431
CACGGAA208300.028.19267533
CGTCTTC200850.028.0357462
Produced by FastQC (version 0.11.5)