FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005309381

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005309381
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28385667
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG63400.041.1802254
CTCGTAT67150.039.03652253
TCTCGTA64000.037.7312752
CGTATGC70400.037.33416455
TATGCCG72500.036.20435757
ATCTCGT75100.032.2011351
ACACGTC93550.028.99249513
ATGCCGT90300.028.25383458
ACGTCTG110250.024.72794715
CCGTCTT108150.023.6887961
CACGTCT115750.023.55288514
CACACGT118650.022.91822212
TATCTCG30650.021.58064350
GATCGGA126050.021.5447671
GCCGTCT120950.021.44158260
GCACACG131750.020.79886211
ATCGGAA133150.020.4230842
TGCCGTC129500.020.2417959
GTCACAC137300.019.88501729
CGTCTGA141400.019.30520416
Produced by FastQC (version 0.11.5)