FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005309386

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005309386
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20467490
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAATGGTCGGTGTAGATCT16890588.252394406935096Illumina Single End PCR Primer 1 (96% over 33bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAATGGTCGGTGTAGATC582210.2844559836110827Illumina Single End PCR Primer 1 (97% over 34bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA1985850.065.2722851
ATCGGAA1986750.064.56112
GCGTCGT1957550.064.4519711
TCGTGTA1945600.064.2134614
AGCGTCG1981850.064.182710
TCGGAAG2006000.064.1651843
CGTCGTG1970900.063.9852112
GTCGTGT1960800.063.8728713
AGAGCGT1997150.063.6147738
AAGAGCG2024500.063.5614367
GAGCGTC2010800.063.3552749
TAGGGAA2004400.063.3168219
CGTGTAG1996750.063.26641515
CGGAAGA2037000.063.023734
GAGTGTA2025000.063.01223427
AAAGAGT1993900.062.95768424
AGAGTGT2030300.062.71221526
ATGGTCG2040900.062.5801234
GTGTAAT2021800.062.54842429
AGTGTAA2027900.062.5466728
Produced by FastQC (version 0.11.5)