FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005309401

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005309401
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33993040
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG77050.032.8871354
CGTATGC81900.030.55499655
CTCGTAT83750.030.2559453
TCTCGTA86400.027.26279352
TATGCCG92750.027.05638557
ACACGTC111100.023.43674313
ATCTCGT101000.023.39117651
ACGTCTG127600.020.67991615
CACGTCT128400.020.33305414
ATGCCGT123700.020.20149458
CGCGGGT93150.019.2861751
CCGTCTT137700.018.02046461
CACACGT149350.017.55152912
GCTTGAA150300.017.28026470
ATCGGAA158250.016.5644532
GATCGGA158700.016.5380271
TATCTCG42250.015.90519850
GCGAACG30350.015.6826413
TGCCGTC169100.015.33690959
GCCGTCT166750.015.09139960
Produced by FastQC (version 0.11.5)