FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005309402

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005309402
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33993040
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCA80850.031.33943461
TCTTACG93400.027.31523733
GCCGTAT97050.026.10758659
TCGCCGT113500.023.24872657
TGGTCGC109350.023.20313854
CGCCGTA115950.022.33493458
GCGTCGT118400.021.87389211
GTATCAT119200.021.84385562
CCGTATC116500.021.8093560
AGCGTCG124000.020.94248810
CTTACGG125200.020.6849434
TACGGGT127150.020.2853836
TCGTGTA126600.020.15250214
GTCGCCG132300.019.70718656
CGTCGTG133650.019.27276612
GATCGGA134600.019.2277181
AAGAGCG136850.019.2058227
AGAGCGT134600.019.111028
GTCGTGT137450.018.53624713
ATCGGAA141100.018.3790282
Produced by FastQC (version 0.11.5)