FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005309405

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005309405
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31314977
Sequences flagged as poor quality0
Sequence length76
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG69400.028.44378354
CGTATGC72200.026.95288755
CTCGTAT73950.026.8355153
TCTCGTA72800.024.37552652
AGTCACG91100.022.3976629
TATGCCG88600.022.12223257
GTCACGT93850.022.0769930
CGCGGGT101450.020.8212011
ATCTCGT87350.020.39543351
ACACGTC101800.020.38607814
TCACGTC107650.019.86452731
GCGAACG30400.018.650173
ACGTCTG111450.018.589516
TAGGTCG23050.018.5238083
CACGTCT112350.018.50288615
GCGGGTC121150.017.4772592
CGGCGAA35750.017.4704991
ATGCCGT111500.017.42103858
GACGGAA127250.016.77720533
ACGGAAC133400.016.08306734
Produced by FastQC (version 0.11.5)