FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005309409

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005309409
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37000381
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG120450.038.9628154
CTCGTAT121800.038.2439953
CGTATGC125500.037.2831955
TCTCGTA114400.037.2303952
TATGCCG129600.036.18525757
ATCTCGT129900.032.84184351
ATGCCGT152350.030.55209758
ACACGTC166100.028.7804913
ACGTCTG179650.026.7266215
CCGTCTT179950.025.67162361
CACGTCT190200.025.09695214
GCCGTCT191700.024.33544560
CACACGT199100.024.09816412
TGCCGTC201400.023.42406559
TATCTCG47850.022.30749950
GCACACG219600.022.18322211
GTCACAC216450.022.15152429
GCTTGAA222650.021.42479770
CGTCTTC216850.021.3192762
GATCGGA229750.020.9441091
Produced by FastQC (version 0.11.5)