FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005309425

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005309425
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34664804
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG78400.041.78278454
CTCGTAT81250.040.3167653
TCTCGTA75950.039.9964452
CGTATGC82450.039.6454955
TATGCCG86300.037.67395457
ATCTCGT90500.033.52769551
ATGCCGT109400.029.30316258
TTAGGAC115750.028.87823735
ACACGTC119600.028.3543413
ACGTCTG133750.025.51171315
ACCTTAG138800.024.76458232
CCGTCTT132950.024.1925561
CCTTAGG140900.023.7727433
TAGGACG28400.023.53983136
CACGTCT145550.023.41928714
CACACGT146750.023.32317412
TATCTCG35250.022.83504750
CACCTTA150550.022.2737731
CTTAGGA154150.022.04700534
GCCGTCT152850.021.13395160
Produced by FastQC (version 0.11.5)