FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005309431

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005309431
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17769875
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTTGAA1010500.062.2038970
TCGTATG1052700.061.350554
CGTATGC1053850.061.2384855
TATGCCG1054700.060.98245657
CTCGTAT1055250.060.97005553
TCTCGTA977150.060.8817452
ATCTCGT980450.060.37507251
ATGCCGT1049500.060.3570858
CCGTCTT1042500.059.92126561
CACCTTA1143800.059.6472131
ACCTTAC1145400.059.6222832
GCCGTCT1057550.059.27356360
TGCCGTC1066850.059.17369559
CTTACCT1153600.059.08942434
CGTCTTC1056600.059.05637762
TATCTCG250650.058.60833750
CCTTACC1168150.058.43132833
ACGTCTG1197250.057.8752215
CAGTCAC1182600.057.8495927
AATCTCG339100.057.8130650
Produced by FastQC (version 0.11.5)