FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005309432

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005309432
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17769875
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGCAATGAGGTGTAGATCT9006565.0684430813385015Illumina Single End PCR Primer 1 (96% over 32bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGCAATGAGGTGTAGATC480220.27024388185060394Illumina Single End PCR Primer 1 (96% over 33bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA1072850.062.503651
GCGTCGT1065800.061.42082211
AGCGTCG1075200.061.25820510
CGTATCA1091850.061.24890561
TCGTGTA1064450.061.05610714
ATCGGAA1094200.061.031722
GCCGTAT1097350.060.86908359
CGTCGTG1074550.060.83272612
AGAGCGT1084650.060.7698638
AAGAGCG1104050.060.6735737
GTCGTGT1075700.060.61717613
TCGCCGT1102500.060.61195857
TGGTCGC1107300.060.5085954
TCGGAAG1109300.060.4809843
CCGTATC1105750.060.3718760
CGCCGTA1110850.060.3215258
GTATCAT1111850.060.28878862
GTCGCCG1117600.060.112456
TAGGGAA1091000.060.11184719
GAGCGTC1100800.059.9577949
Produced by FastQC (version 0.11.5)