FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005309433

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005309433
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33732973
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATACGCG80750.040.82956335
TCGTATG80200.040.49847454
CAATACG86800.038.22441533
CGTATGC85100.038.08434355
CTCGTAT89150.036.3930953
TCTCGTA88200.033.92889452
TACGCGT29050.033.01191736
TATGCCG99350.032.65733757
AATACGC103050.032.29996534
ATCTCGT106050.028.6472251
ACACGTC118450.028.24579213
CACAATA119650.027.78795831
ACGTCTG129600.025.97741115
CACGTCT130950.025.81654214
GTCACAA130750.025.75018729
ATGCCGT124850.025.53794158
ACAATAC133800.024.98036832
TACGCGC33300.024.06897436
ACGCGTA15900.022.89299237
TATCTCG39900.022.71927850
Produced by FastQC (version 0.11.5)