FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005309434

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005309434
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33732973
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTGCGATAGGTGTAGATCT408440.12108034474162713Illumina Single End PCR Primer 1 (97% over 34bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCA86550.037.80724361
CGATAGG87500.037.55653435
TGCGATA87650.037.45199633
GCGATAG94300.035.10799834
GTTGCGA96100.034.26761231
GCCGTAT104100.031.46641359
TTGCGAT104550.031.46501232
TGGTCGC112850.029.27505554
TCGCCGT116350.029.02576657
GTATCAT118250.027.81996362
GATAGGT120900.027.38402636
CGCCGTA126150.026.2992458
GCGTCGT130150.025.68071411
AGCGTCG133350.025.09069810
CCGTATC132650.024.77354860
GTCGCCG138150.024.06589156
TCGTGTA137350.023.90079714
AGAGCGT144750.023.0419738
ATAGGTG152950.022.08060337
AAGAGCG152900.021.8593227
Produced by FastQC (version 0.11.5)