FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005309438

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005309438
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30536166
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGCATTGGTGTGTAGATCT1050920.34415584458114357Illumina Single End PCR Primer 1 (96% over 32bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGCATTGGTGTGTAGATC846010.2770518080102132Illumina Single End PCR Primer 1 (96% over 33bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGATCGG210800.032.693981
CGTATCA252400.032.14026361
GCCGTAT265900.030.6137659
TCGCCGT266600.030.40206157
CGCCGTA271900.029.7965858
GTCGCCG279350.029.18961156
CGTCGTG276800.028.73856712
GCGTCGT279550.028.54381811
AGCGTCG282100.028.44763810
CCGTATC286300.028.35916160
TGGTCGC287650.028.26219654
GTATCAT297700.027.26129762
AGAGCGT298650.026.7892828
GAGCGTC302800.026.5491339
TCGTGTA298700.026.44505514
GATCGGA308350.026.220611
GTCGTGT303000.026.06886313
AAGAGCG311950.025.792977
ATCGGAA312700.025.7306862
GGTCGCC317850.025.70903455
Produced by FastQC (version 0.11.5)