FastQCFastQC Report
Tue 31 May 2022
EGAF00005309582

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005309582
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences123165184
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAGAATTCACCCCACCAGTGCAGGCTGCCTATCAGAAAGTGGTGGCTGG4324480.35111221041166957No Hit
GGCTAATGCCCTGGCCCACAAGTATCACTAAGCTCGCTTTCTTGCTGTCC4085540.33171224751306344No Hit
GGCTGGTGTGGCTAATGCCCTGGCCCACAAGTATCACTAAGCTCGCTTTC3392370.2754325443138217No Hit
CTAATGCCCTGGCCCACAAGTATCACTAAGCTCGCTTTCTTGCTGTCCAA3274190.26583730025524094No Hit
GGTGGCTGGTGTGGCTAATGCCCTGGCCCACAAGTATCACTAAGCTCGCT3232860.2624816441633376No Hit
CAAAGAATTCACCCCACCAGTGCAGGCTGCCTATCAGAAAGTGGTGGCTG3148260.2556128199345685No Hit
GTTCACCCCTGCGGTGCACGCCTCCCTGGACAAGTTCCTGGCTTCTGTGA3037420.2466135235100205No Hit
AGAAAGTGGTGGCTGGTGTGGCTAATGCCCTGGCCCACAAGTATCACTAA2853020.23164176006102502No Hit
GTGGCTAATGCCCTGGCCCACAAGTATCACTAAGCTCGCTTTCTTGCTGT2690000.21840587677764522No Hit
CCCACAAGTATCACTAAGCTCGCTTTCTTGCTGTCCAATTTCTATTAAAG2620240.21274193850106213No Hit
CCCTGGCCCACAAGTATCACTAAGCTCGCTTTCTTGCTGTCCAATTTCTA2594580.21065855753522036No Hit
CTGGTGTGGCTAATGCCCTGGCCCACAAGTATCACTAAGCTCGCTTTCTT2473430.20082217390265095No Hit
CTGCGGTGCACGCCTCCCTGGACAAGTTCCTGGCTTCTGTGAGCACCGTG2433710.19759723656971115No Hit
CACAAGTATCACTAAGCTCGCTTTCTTGCTGTCCAATTTCTATTAAAGGT2381180.1933322325893655No Hit
GTTCCTGGCTTCTGTGAGCACCGTGCTGACCTCCAAATACCGTTAAGCTG2375240.1928499534413881No Hit
CCGCCGAGTTCACCCCTGCGGTGCACGCCTCCCTGGACAAGTTCCTGGCT2336120.18967373117390057No Hit
ATTCACCCCACCAGTGCAGGCTGCCTATCAGAAAGTGGTGGCTGGTGTGG2329400.1891281224408352No Hit
CTGGCCCACAAGTATCACTAAGCTCGCTTTCTTGCTGTCCAATTTCTATT2315230.18797763497840428No Hit
CAGAAAGTGGTGGCTGGTGTGGCTAATGCCCTGGCCCACAAGTATCACTA2271100.18439464191439037No Hit
GTGGCTGGTGTGGCTAATGCCCTGGCCCACAAGTATCACTAAGCTCGCTT2253440.18296079515457875No Hit
CTCGCTTTCTTGCTGTCCAATTTCTATTAAAGGTTCCTTTGTTCCCTAAG2245410.18230882519527597No Hit
CTGGCTTCTGTGAGCACCGTGCTGACCTCCAAATACCGTTAAGCTGGAGC2245200.18229177492236767No Hit
CCCTGGCCGCCCACCTCCCCGCCGAGTTCACCCCTGCGGTGCACGCCTCC2231820.18120542896278222No Hit
GCCCTGGCCCACAAGTATCACTAAGCTCGCTTTCTTGCTGTCCAATTTCT2156250.17506976646906972No Hit
ATCAGAAAGTGGTGGCTGGTGTGGCTAATGCCCTGGCCCACAAGTATCAC2135720.17340289931284475No Hit
GCAAAGAATTCACCCCACCAGTGCAGGCTGCCTATCAGAAAGTGGTGGCT2112550.17152168586862987No Hit
CTTCTGTGAGCACCGTGCTGACCTCCAAATACCGTTAAGCTGGAGCCTCG2112410.17151031902002434No Hit
GTCCAATTTCTATTAAAGGTTCCTTTGTTCCCTAAGTCCAACTACTAAAC2079350.1688261189136047No Hit
CTTTGGCAAAGAATTCACCCCACCAGTGCAGGCTGCCTATCAGAAAGTGG2031370.1649305375129387No Hit
CCTGGCTTCTGTGAGCACCGTGCTGACCTCCAAATACCGTTAAGCTGGAG2019270.1639481170263181No Hit
CGCCGAGTTCACCCCTGCGGTGCACGCCTCCCTGGACAAGTTCCTGGCTT2003940.16270344710401277No Hit
CACCAGTGCAGGCTGCCTATCAGAAAGTGGTGGCTGGTGTGGCTAATGCC1980360.16078894503173882No Hit
CCACAAGTATCACTAAGCTCGCTTTCTTGCTGTCCAATTTCTATTAAAGG1954290.15867227543783802No Hit
CCCTGGACAAGTTCCTGGCTTCTGTGAGCACCGTGCTGACCTCCAAATAC1869460.15178477710064558No Hit
GCCGAGTTCACCCCTGCGGTGCACGCCTCCCTGGACAAGTTCCTGGCTTC1869070.15175311230810162No Hit
CTTCAAGCTCCTAAGCCACTGCCTGCTGGTGACCCTGGCCGCCCACCTCC1857710.15083077373553877No Hit
GGCTGCCTATCAGAAAGTGGTGGCTGGTGTGGCTAATGCCCTGGCCCACA1849310.1501487628192071No Hit
GTGCAGGCTGCCTATCAGAAAGTGGTGGCTGGTGTGGCTAATGCCCTGGC1792500.14553625803863532No Hit
CTTCAGGCTCCTGGGCAACGTGCTGGTCTGTGTGCTGGCCCATCACTTTG1754690.14246639699738523No Hit
CCCCACCAGTGCAGGCTGCCTATCAGAAAGTGGTGGCTGGTGTGGCTAAT1753890.14240144357678222No Hit
CCAATTTCTATTAAAGGTTCCTTTGTTCCCTAAGTCCAACTACTAAACTG1689280.13715564294533106No Hit
CACGCCTCCCTGGACAAGTTCCTGGCTTCTGTGAGCACCGTGCTGACCTC1672060.13575752056685111No Hit
GGTGTGGCTAATGCCCTGGCCCACAAGTATCACTAAGCTCGCTTTCTTGC1671670.13572585577430712No Hit
CATCACTTTGGCAAAGAATTCACCCCACCAGTGCAGGCTGCCTATCAGAA1653660.1342635918929817No Hit
CTGGCCCATCACTTTGGCAAAGAATTCACCCCACCAGTGCAGGCTGCCTA1620800.13159563014171277No Hit
CTATCAGAAAGTGGTGGCTGGTGTGGCTAATGCCCTGGCCCACAAGTATC1609440.13067329156914992No Hit
CCTGGCCGCCCACCTCCCCGCCGAGTTCACCCCTGCGGTGCACGCCTCCC1607090.13048249089612857No Hit
CCCACCTCCCCGCCGAGTTCACCCCTGCGGTGCACGCCTCCCTGGACAAG1591350.1292045323457642No Hit
CAAGTTCCTGGCTTCTGTGAGCACCGTGCTGACCTCCAAATACCGTTAAG1585590.1287368677174225No Hit
GCCTATCAGAAAGTGGTGGCTGGTGTGGCTAATGCCCTGGCCCACAAGTA1579810.12826757925356566No Hit
GGACAAGTTCCTGGCTTCTGTGAGCACCGTGCTGACCTCCAAATACCGTT1560390.12669083496842742No Hit
CCCCTGCGGTGCACGCCTCCCTGGACAAGTTCCTGGCTTCTGTGAGCACC1530120.12423315991636077No Hit
CACTAAGCTCGCTTTCTTGCTGTCCAATTTCTATTAAAGGTTCCTTTGTT1525290.12384100363947008No Hit
CCTCCAAATACCGTTAAGCTGGAGCCTCGGTAGCCGTTCCTCCTGCCCGC1457910.11837030178918095No Hit
CCCTGCGGTGCACGCCTCCCTGGACAAGTTCCTGGCTTCTGTGAGCACCG1454010.11805365386374123No Hit
CTTTCTTGCTGTCCAATTTCTATTAAAGGTTCCTTTGTTCCCTAAGTCCA1445210.11733916623710805No Hit
CGCCCACCTCCCCGCCGAGTTCACCCCTGCGGTGCACGCCTCCCTGGACA1429850.11609206056153011No Hit
CTGCTGGTGACCCTGGCCGCCCACCTCCCCGCCGAGTTCACCCCTGCGGT1424010.11561790059112809No Hit
ATTAAAGGTTCCTTTGTTCCCTAAGTCCAACTACTAAACTGGGGGATATT1412120.11465253037741575No Hit
CTCCAAATACCGTTAAGCTGGAGCCTCGGTAGCCGTTCCTCCTGCCCGCT1410290.11450394942778634No Hit
CCTGGACAAGTTCCTGGCTTCTGTGAGCACCGTGCTGACCTCCAAATACC1399550.11363194975619083No Hit
GCACGCCTCCCTGGACAAGTTCCTGGCTTCTGTGAGCACCGTGCTGACCT1394320.11320731676899862No Hit
GCTGCCTATCAGAAAGTGGTGGCTGGTGTGGCTAATGCCCTGGCCCACAA1385960.11252855352369708No Hit
CCTCCCTGGACAAGTTCCTGGCTTCTGTGAGCACCGTGCTGACCTCCAAA1364350.1107739992496581No Hit
GTGCTGGTCTGTGTGCTGGCCCATCACTTTGGCAAAGAATTCACCCCACC1358450.11029496777271083No Hit
GCTAATGCCCTGGCCCACAAGTATCACTAAGCTCGCTTTCTTGCTGTCCA1343760.10910226058688792No Hit
GTCAACTTCAAGCTCCTAAGCCACTGCCTGCTGGTGACCCTGGCCGCCCA1309760.1063417402112597No Hit
CTAAGCTCGCTTTCTTGCTGTCCAATTTCTATTAAAGGTTCCTTTGTTCC1305940.1060315876278803No Hit
GGTCTGTGTGCTGGCCCATCACTTTGGCAAAGAATTCACCCCACCAGTGC1300210.10556635875281117No Hit
CTGACCTCCAAATACCGTTAAGCTGGAGCCTCGGTAGCCGTTCCTCCTGC1299250.10548841464808757No Hit
CCCACCAGTGCAGGCTGCCTATCAGAAAGTGGTGGCTGGTGTGGCTAATG1294320.10508813919362146No Hit
CACCCCTGCGGTGCACGCCTCCCTGGACAAGTTCCTGGCTTCTGTGAGCA1291130.10482913742896693No Hit
GAAAGTGGTGGCTGGTGTGGCTAATGCCCTGGCCCACAAGTATCACTAAG1279560.1038897485834958No Hit
CTGAGAACTTCAGGCTCCTGGGCAACGTGCTGGTCTGTGTGCTGGCCCAT1278340.10379069461707621No Hit
CACCCCACCAGTGCAGGCTGCCTATCAGAAAGTGGTGGCTGGTGTGGCTA1268370.1029812126128111No Hit
TTCACCCCTGCGGTGCACGCCTCCCTGGACAAGTTCCTGGCTTCTGTGAG1250840.1015579207838475No Hit
CCTGGCCCACAAGTATCACTAAGCTCGCTTTCTTGCTGTCCAATTTCTAT1245410.10111704944150451No Hit
TGTGGCTAATGCCCTGGCCCACAAGTATCACTAAGCTCGCTTTCTTGCTG1243670.10097577575169295No Hit
GTGCTGGCCCATCACTTTGGCAAAGAATTCACCCCACCAGTGCAGGCTGC1234620.1002409901811213No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)