FastQCFastQC Report
Fri 27 May 2022
EGAF00005309608

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005309608
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences238733957
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAGAATTCACCCCACCAGTGCAGGCTGCCTATCAGAAAGTGGTGGCTGG12694460.5317408616487683No Hit
CAAAGAATTCACCCCACCAGTGCAGGCTGCCTATCAGAAAGTGGTGGCTG9338870.39118314450759095No Hit
CTTCAAGCTCCTAAGCCACTGCCTGCTGGTGACCCTGGCCGCCCACCTCC7749820.32462160378801913No Hit
CTTCAGGCTCCTGGGCAACGTGCTGGTCTGTGTGCTGGCCCATCACTTTG7620890.31922103146809566No Hit
GTTCACCCCTGCGGTGCACGCCTCCCTGGACAAGTTCCTGGCTTCTGTGA7278350.30487284219898386No Hit
CCCTGGCCGCCCACCTCCCCGCCGAGTTCACCCCTGCGGTGCACGCCTCC6772170.28367016092310654No Hit
CCGCCGAGTTCACCCCTGCGGTGCACGCCTCCCTGGACAAGTTCCTGGCT6347430.26587880835066957No Hit
CTTTGGCAAAGAATTCACCCCACCAGTGCAGGCTGCCTATCAGAAAGTGG6306960.2641836159068062No Hit
ATTCACCCCACCAGTGCAGGCTGCCTATCAGAAAGTGGTGGCTGGTGTGG6302880.2640127143705828No Hit
GGCTGGTGTGGCTAATGCCCTGGCCCACAAGTATCACTAAGCTCGCTTTC6222690.2606537452064266No Hit
GCAAAGAATTCACCCCACCAGTGCAGGCTGCCTATCAGAAAGTGGTGGCT6189330.25925637382201144No Hit
GGCTAATGCCCTGGCCCACAAGTATCACTAAGCTCGCTTTCTTGCTGTCC6115490.25616339111741865No Hit
GGTGGCTGGTGTGGCTAATGCCCTGGCCCACAAGTATCACTAAGCTCGCT5874570.24607182295395036No Hit
CTGAGAACTTCAGGCTCCTGGGCAACGTGCTGGTCTGTGTGCTGGCCCAT5770340.24170587513028152No Hit
GTCAACTTCAAGCTCCTAAGCCACTGCCTGCTGGTGACCCTGGCCGCCCA5687860.23825098329015676No Hit
AGAAAGTGGTGGCTGGTGTGGCTAATGCCCTGGCCCACAAGTATCACTAA5515960.23105049944780165No Hit
CTGGCCCATCACTTTGGCAAAGAATTCACCCCACCAGTGCAGGCTGCCTA5343970.22384624571861805No Hit
GTGCTGGTCTGTGTGCTGGCCCATCACTTTGGCAAAGAATTCACCCCACC5274760.2209472027475337No Hit
CATCACTTTGGCAAAGAATTCACCCCACCAGTGCAGGCTGCCTATCAGAA5231250.21912467190413135No Hit
CACCAGTGCAGGCTGCCTATCAGAAAGTGGTGGCTGGTGTGGCTAATGCC5076740.2126526139722972No Hit
CTGCGGTGCACGCCTCCCTGGACAAGTTCCTGGCTTCTGTGAGCACCGTG5056600.2118089970753511No Hit
CGCCGAGTTCACCCCTGCGGTGCACGCCTCCCTGGACAAGTTCCTGGCTT5023060.21040408591727905No Hit
CTAATGCCCTGGCCCACAAGTATCACTAAGCTCGCTTTCTTGCTGTCCAA4960090.2077664217663011No Hit
CAGAAAGTGGTGGCTGGTGTGGCTAATGCCCTGGCCCACAAGTATCACTA4875710.20423194342646445No Hit
CCTGGCCGCCCACCTCCCCGCCGAGTTCACCCCTGCGGTGCACGCCTCCC4862060.20366017725748164No Hit
ATCAGAAAGTGGTGGCTGGTGTGGCTAATGCCCTGGCCCACAAGTATCAC4757640.19928627078384162No Hit
GCCGAGTTCACCCCTGCGGTGCACGCCTCCCTGGACAAGTTCCTGGCTTC4734970.19833667817938444No Hit
CTGCTGGTGACCCTGGCCGCCCACCTCCCCGCCGAGTTCACCCCTGCGGT4729890.19812388901173367No Hit
GTGGCTAATGCCCTGGCCCACAAGTATCACTAAGCTCGCTTTCTTGCTGT4393160.18401906688121455No Hit
CCCCACCAGTGCAGGCTGCCTATCAGAAAGTGGTGGCTGGTGTGGCTAAT4358430.18256430944174398No Hit
GTGCTGGCCCATCACTTTGGCAAAGAATTCACCCCACCAGTGCAGGCTGC4344670.18198793563330415No Hit
GTGCAGGCTGCCTATCAGAAAGTGGTGGCTGGTGTGGCTAATGCCCTGGC4343930.18195693878604793No Hit
GGTCTGTGTGCTGGCCCATCACTTTGGCAAAGAATTCACCCCACCAGTGC4321060.1809989686553053No Hit
CCCACCTCCCCGCCGAGTTCACCCCTGCGGTGCACGCCTCCCTGGACAAG4309140.1804996680886917No Hit
GCAACGTGCTGGTCTGTGTGCTGGCCCATCACTTTGGCAAAGAATTCACC4298960.18007325200076166No Hit
ATCACTTTGGCAAAGAATTCACCCCACCAGTGCAGGCTGCCTATCAGAAA4209750.17633645640113108No Hit
CGCCCACCTCCCCGCCGAGTTCACCCCTGCGGTGCACGCCTCCCTGGACA4174080.1748423245881188No Hit
GCCACTGCCTGCTGGTGACCCTGGCCGCCCACCTCCCCGCCGAGTTCACC4149130.17379722818400736No Hit
CTGGTGTGGCTAATGCCCTGGCCCACAAGTATCACTAAGCTCGCTTTCTT4143550.17356349520064293No Hit
GTGGCTGGTGTGGCTAATGCCCTGGCCCACAAGTATCACTAAGCTCGCTT4008670.16791369147372695No Hit
CTCCTGGGCAACGTGCTGGTCTGTGTGCTGGCCCATCACTTTGGCAAAGA3986310.16697708403501224No Hit
CTGGTCTGTGTGCTGGCCCATCACTTTGGCAAAGAATTCACCCCACCAGT3984070.16688325574061505No Hit
GGCTGCCTATCAGAAAGTGGTGGCTGGTGTGGCTAATGCCCTGGCCCACA3983280.1668501645117875No Hit
GAGAACTTCAGGCTCCTGGGCAACGTGCTGGTCTGTGTGCTGGCCCATCA3931000.16466027914076756No Hit
GACCCGGTCAACTTCAAGCTCCTAAGCCACTGCCTGCTGGTGACCCTGGC3877030.16239960367263548No Hit
CAAGCTTCGGGTGGACCCGGTCAACTTCAAGCTCCTAAGCCACTGCCTGC3851110.16131387626603952No Hit
GCTGGCCCATCACTTTGGCAAAGAATTCACCCCACCAGTGCAGGCTGCCT3826040.16026375334615678No Hit
GTTCCTGGCTTCTGTGAGCACCGTGCTGACCTCCAAATACCGTTAAGCTG3805830.15941720431501077No Hit
GGCCCATCACTTTGGCAAAGAATTCACCCCACCAGTGCAGGCTGCCTATC3788340.1586845896413471No Hit
CTTCGGGTGGACCCGGTCAACTTCAAGCTCCTAAGCCACTGCCTGCTGGT3735630.15647669258881342No Hit
CTGGCTTCTGTGAGCACCGTGCTGACCTCCAAATACCGTTAAGCTGGAGC3626630.1519109407632363No Hit
CTAAGCCACTGCCTGCTGGTGACCCTGGCCGCCCACCTCCCCGCCGAGTT3599580.15077788033312747No Hit
CCGCCCACCTCCCCGCCGAGTTCACCCCTGCGGTGCACGCCTCCCTGGAC3572980.14966366933716094No Hit
CCCTGGACAAGTTCCTGGCTTCTGTGAGCACCGTGCTGACCTCCAAATAC3460640.1449580128226166No Hit
GCCTATCAGAAAGTGGTGGCTGGTGTGGCTAATGCCCTGGCCCACAAGTA3418500.14319286803426964No Hit
GTGTGCTGGCCCATCACTTTGGCAAAGAATTCACCCCACCAGTGCAGGCT3416970.14312877995818585No Hit
CCCACCAGTGCAGGCTGCCTATCAGAAAGTGGTGGCTGGTGTGGCTAATG3415660.14307390716101606No Hit
CTGGCCGCCCACCTCCCCGCCGAGTTCACCCCTGCGGTGCACGCCTCCCT3405340.14264162680468617No Hit
GCCTGCTGGTGACCCTGGCCGCCCACCTCCCCGCCGAGTTCACCCCTGCG3340010.13990510784353982No Hit
CTATCAGAAAGTGGTGGCTGGTGTGGCTAATGCCCTGGCCCACAAGTATC3337380.139794943372886No Hit
CCCTGGCCCACAAGTATCACTAAGCTCGCTTTCTTGCTGTCCAATTTCTA3330080.13948916366346661No Hit
GTGACCCTGGCCGCCCACCTCCCCGCCGAGTTCACCCCTGCGGTGCACGC3298870.13818185068662017No Hit
CGTGGATCCTGAGAACTTCAGGCTCCTGGGCAACGTGCTGGTCTGTGTGC3297370.1381190192394792No Hit
CACGCCTCCCTGGACAAGTTCCTGGCTTCTGTGAGCACCGTGCTGACCTC3279500.13737048726587311No Hit
CCCCTGCGGTGCACGCCTCCCTGGACAAGTTCCTGGCTTCTGTGAGCACC3256320.136399531969388No Hit
CACCCCACCAGTGCAGGCTGCCTATCAGAAAGTGGTGGCTGGTGTGGCTA3249840.13612810011773901No Hit
GCCGCCCACCTCCCCGCCGAGTTCACCCCTGCGGTGCACGCCTCCCTGGA3203660.13419372929842568No Hit
CCTGGCTTCTGTGAGCACCGTGCTGACCTCCAAATACCGTTAAGCTGGAG3202210.1341329922328561No Hit
CCCACAAGTATCACTAAGCTCGCTTTCTTGCTGTCCAATTTCTATTAAAG3184250.13338069037242156No Hit
GCTGCCTATCAGAAAGTGGTGGCTGGTGTGGCTAATGCCCTGGCCCACAA3158110.1322857476869116No Hit
GGTGACCCTGGCCGCCCACCTCCCCGCCGAGTTCACCCCTGCGGTGCACG3147260.13183126688592522No Hit
CTTCTGTGAGCACCGTGCTGACCTCCAAATACCGTTAAGCTGGAGCCTCG3109520.13025042767585845No Hit
CTGGCCCACAAGTATCACTAAGCTCGCTTTCTTGCTGTCCAATTTCTATT3098330.1297817050801868No Hit
GTCCGCCCTGAGCGACCTGCACGCGCACAAGCTTCGGGTGGACCCGGTCA3080040.12901558030138127No Hit
CCCTGCGGTGCACGCCTCCCTGGACAAGTTCCTGGCTTCTGTGAGCACCG3074460.12878184731801684No Hit
TGAGAACTTCAGGCTCCTGGGCAACGTGCTGGTCTGTGTGCTGGCCCATC3037490.12723326158414908No Hit
GCCCTGGCCCACAAGTATCACTAAGCTCGCTTTCTTGCTGTCCAATTTCT2965970.12423745818446767No Hit
CACAAGTATCACTAAGCTCGCTTTCTTGCTGTCCAATTTCTATTAAAGGT2921170.12236089229652404No Hit
GCAGGCTGCCTATCAGAAAGTGGTGGCTGGTGTGGCTAATGCCCTGGCCC2902190.12156586505203362No Hit
TTCACCCCTGCGGTGCACGCCTCCCTGGACAAGTTCCTGGCTTCTGTGAG2874630.12041144192989689No Hit
CACCCCTGCGGTGCACGCCTCCCTGGACAAGTTCCTGGCTTCTGTGAGCA2872440.12031970801707106No Hit
GCACGCCTCCCTGGACAAGTTCCTGGCTTCTGTGAGCACCGTGCTGACCT2838440.11889552854854243No Hit
CGAGTTCACCCCTGCGGTGCACGCCTCCCTGGACAAGTTCCTGGCTTCTG2837660.11886285619602911No Hit
CCACTGCCTGCTGGTGACCCTGGCCGCCCACCTCCCCGCCGAGTTCACCC2735310.11457565711944363No Hit
AGAATTCACCCCACCAGTGCAGGCTGCCTATCAGAAAGTGGTGGCTGGTG2733660.11450654252758857No Hit
CTCCTAAGCCACTGCCTGCTGGTGACCCTGGCCGCCCACCTCCCCGCCGA2727710.11425731112059605No Hit
CCATCACTTTGGCAAAGAATTCACCCCACCAGTGCAGGCTGCCTATCAGA2726010.11418610214716962No Hit
TGGGCAACGTGCTGGTCTGTGTGCTGGCCCATCACTTTGGCAAAGAATTC2720140.1139402217506913No Hit
GTGACAAGCTGCACGTGGATCCTGAGAACTTCAGGCTCCTGGGCAACGTG2692060.11276401706021234No Hit
TCAACTTCAAGCTCCTAAGCCACTGCCTGCTGGTGACCCTGGCCGCCCAC2673000.11196563880520775No Hit
GGTGGACCCGGTCAACTTCAAGCTCCTAAGCCACTGCCTGCTGGTGACCC2669440.11181651883732653No Hit
CAAGCTCCTAAGCCACTGCCTGCTGGTGACCCTGGCCGCCCACCTCCCCG2636180.1104233362160541No Hit
CTGTGTGCTGGCCCATCACTTTGGCAAAGAATTCACCCCACCAGTGCAGG2631330.11022018120363161No Hit
CAAGTTCCTGGCTTCTGTGAGCACCGTGCTGACCTCCAAATACCGTTAAG2618040.10966349458196263No Hit
GGACAAGTTCCTGGCTTCTGTGAGCACCGTGCTGACCTCCAAATACCGTT2598290.1088362138612732No Hit
CAACTTCAAGCTCCTAAGCCACTGCCTGCTGGTGACCCTGGCCGCCCACC2594340.10867075771713532No Hit
GCTGGTCTGTGTGCTGGCCCATCACTTTGGCAAAGAATTCACCCCACCAG2579570.10805207740095389No Hit
GGTGTGGCTAATGCCCTGGCCCACAAGTATCACTAAGCTCGCTTTCTTGC2572290.10774713544416306No Hit
GAATTCACCCCACCAGTGCAGGCTGCCTATCAGAAAGTGGTGGCTGGTGT2552690.10692613786818772No Hit
GGCAAAGAATTCACCCCACCAGTGCAGGCTGCCTATCAGAAAGTGGTGGC2534390.1061595942130679No Hit
CCTGGACAAGTTCCTGGCTTCTGTGAGCACCGTGCTGACCTCCAAATACC2518370.10548855435760233No Hit
AAGAATTCACCCCACCAGTGCAGGCTGCCTATCAGAAAGTGGTGGCTGGT2502150.10480913697585133No Hit
GGTCAACTTCAAGCTCCTAAGCCACTGCCTGCTGGTGACCCTGGCCGCCC2500440.10473750912611063No Hit
CCTCCCTGGACAAGTTCCTGGCTTCTGTGAGCACCGTGCTGACCTCCAAA2496850.10458713252928656No Hit
TCTGTGTGCTGGCCCATCACTTTGGCAAAGAATTCACCCCACCAGTGCAG2455220.10284335043296752No Hit
GAAAGTGGTGGCTGGTGTGGCTAATGCCCTGGCCCACAAGTATCACTAAG2434650.10198172185450768No Hit
GGGCAACGTGCTGGTCTGTGTGCTGGCCCATCACTTTGGCAAAGAATTCA2425210.10158630261383386No Hit
CTCAAGGGCACCTTTGCCACACTGAGTGAGCTGCACTGTGACAAGCTGCA2415630.10118501910476019No Hit
TTCACCCCACCAGTGCAGGCTGCCTATCAGAAAGTGGTGGCTGGTGTGGC2413120.10107988114987765No Hit
GCCCACCTCCCCGCCGAGTTCACCCCTGCGGTGCACGCCTCCCTGGACAA2407970.10086415984802699No Hit
TGCTGGCCCATCACTTTGGCAAAGAATTCACCCCACCAGTGCAGGCTGCC2404290.10071001336437446No Hit
CCACAAGTATCACTAAGCTCGCTTTCTTGCTGTCCAATTTCTATTAAAGG2402450.10063294012254821No Hit
CGCCTCCCTGGACAAGTTCCTGGCTTCTGTGAGCACCGTGCTGACCTCCA2388740.10005866069567974No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)