FastQCFastQC Report
Tue 5 Jul 2022
EGAF00005309610

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005309610
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences181103110
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAGAATTCACCCCACCAGTGCAGGCTGCCTATCAGAAAGTGGTGGCTGG9554560.5275756998319907No Hit
CAAAGAATTCACCCCACCAGTGCAGGCTGCCTATCAGAAAGTGGTGGCTG7211880.3982195556995128No Hit
CTTCAGGCTCCTGGGCAACGTGCTGGTCTGTGTGCTGGCCCATCACTTTG5876680.3244935992540382No Hit
CTTCAAGCTCCTAAGCCACTGCCTGCTGGTGACCCTGGCCGCCCACCTCC5830040.3219182707574707No Hit
GTTCACCCCTGCGGTGCACGCCTCCCTGGACAAGTTCCTGGCTTCTGTGA5582580.30825423152589704No Hit
CCCTGGCCGCCCACCTCCCCGCCGAGTTCACCCCTGCGGTGCACGCCTCC5146010.2841480745416244No Hit
CCGCCGAGTTCACCCCTGCGGTGCACGCCTCCCTGGACAAGTTCCTGGCT4846870.2676304123104236No Hit
ATTCACCCCACCAGTGCAGGCTGCCTATCAGAAAGTGGTGGCTGGTGTGG4780330.2639562622640771No Hit
CTTTGGCAAAGAATTCACCCCACCAGTGCAGGCTGCCTATCAGAAAGTGG4742750.2618812012670572No Hit
GCAAAGAATTCACCCCACCAGTGCAGGCTGCCTATCAGAAAGTGGTGGCT4707870.2599552266109621No Hit
GGCTGGTGTGGCTAATGCCCTGGCCCACAAGTATCACTAAGCTCGCTTTC4628690.2555831316204344No Hit
GGCTAATGCCCTGGCCCACAAGTATCACTAAGCTCGCTTTCTTGCTGTCC4482740.24752418663599982No Hit
CTGAGAACTTCAGGCTCCTGGGCAACGTGCTGGTCTGTGTGCTGGCCCAT4480140.24738062201140554No Hit
GTCAACTTCAAGCTCCTAAGCCACTGCCTGCTGGTGACCCTGGCCGCCCA4345670.23995557006171786No Hit
GGTGGCTGGTGTGGCTAATGCCCTGGCCCACAAGTATCACTAAGCTCGCT4322900.23869827525325213No Hit
AGAAAGTGGTGGCTGGTGTGGCTAATGCCCTGGCCCACAAGTATCACTAA4092820.22599391031992772No Hit
CTGGCCCATCACTTTGGCAAAGAATTCACCCCACCAGTGCAGGCTGCCTA4057470.22404198359707905No Hit
GTGCTGGTCTGTGTGCTGGCCCATCACTTTGGCAAAGAATTCACCCCACC4036700.22289512311522425No Hit
CATCACTTTGGCAAAGAATTCACCCCACCAGTGCAGGCTGCCTATCAGAA3908740.21582953489865525No Hit
CTGCGGTGCACGCCTCCCTGGACAAGTTCCTGGCTTCTGTGAGCACCGTG3872410.21382349535576722No Hit
CGCCGAGTTCACCCCTGCGGTGCACGCCTCCCTGGACAAGTTCCTGGCTT3858810.2130725419348127No Hit
CACCAGTGCAGGCTGCCTATCAGAAAGTGGTGGCTGGTGTGGCTAATGCC3820530.210958828923479No Hit
CCTGGCCGCCCACCTCCCCGCCGAGTTCACCCCTGCGGTGCACGCCTCCC3710640.20489101484783998No Hit
CTAATGCCCTGGCCCACAAGTATCACTAAGCTCGCTTTCTTGCTGTCCAA3631590.20052609808854194No Hit
CTGCTGGTGACCCTGGCCGCCCACCTCCCCGCCGAGTTCACCCCTGCGGT3628020.20032897281554138No Hit
CAGAAAGTGGTGGCTGGTGTGGCTAATGCCCTGGCCCACAAGTATCACTA3626880.20026602524937315No Hit
GCCGAGTTCACCCCTGCGGTGCACGCCTCCCTGGACAAGTTCCTGGCTTC3617000.19972047967591502No Hit
ATCAGAAAGTGGTGGCTGGTGTGGCTAATGCCCTGGCCCACAAGTATCAC3514390.19405464654913987No Hit
CCCCACCAGTGCAGGCTGCCTATCAGAAAGTGGTGGCTGGTGTGGCTAAT3323860.1835341204245471No Hit
GTGCAGGCTGCCTATCAGAAAGTGGTGGCTGGTGTGGCTAATGCCCTGGC3317180.1831652697736665No Hit
GTGCTGGCCCATCACTTTGGCAAAGAATTCACCCCACCAGTGCAGGCTGC3312820.18292452294165462No Hit
CCCACCTCCCCGCCGAGTTCACCCCTGCGGTGCACGCCTCCCTGGACAAG3298970.18215976522987376No Hit
GCAACGTGCTGGTCTGTGTGCTGGCCCATCACTTTGGCAAAGAATTCACC3289320.18162691960397587No Hit
GGTCTGTGTGCTGGCCCATCACTTTGGCAAAGAATTCACCCCACCAGTGC3273690.18076387534151125No Hit
CGCCCACCTCCCCGCCGAGTTCACCCCTGCGGTGCACGCCTCCCTGGACA3210570.17727856799366948No Hit
GTGGCTAATGCCCTGGCCCACAAGTATCACTAAGCTCGCTTTCTTGCTGT3182250.17571481792885832No Hit
ATCACTTTGGCAAAGAATTCACCCCACCAGTGCAGGCTGCCTATCAGAAA3170490.17506546408838589No Hit
GCCACTGCCTGCTGGTGACCCTGGCCGCCCACCTCCCCGCCGAGTTCACC3160370.17450666639573445No Hit
CTGGTGTGGCTAATGCCCTGGCCCACAAGTATCACTAAGCTCGCTTTCTT3135560.17313672857412554No Hit
CTCCTGGGCAACGTGCTGGTCTGTGTGCTGGCCCATCACTTTGGCAAAGA3051450.16849241296850176No Hit
GAGAACTTCAGGCTCCTGGGCAACGTGCTGGTCTGTGTGCTGGCCCATCA3041400.1679374804772817No Hit
CTGGTCTGTGTGCTGGCCCATCACTTTGGCAAAGAATTCACCCCACCAGT3031590.16739580010525495No Hit
GGCTGCCTATCAGAAAGTGGTGGCTGGTGTGGCTAATGCCCTGGCCCACA3001570.1657381808628245No Hit
CAAGCTTCGGGTGGACCCGGTCAACTTCAAGCTCCTAAGCCACTGCCTGC2932960.16194973128843562No Hit
GCTGGCCCATCACTTTGGCAAAGAATTCACCCCACCAGTGCAGGCTGCCT2927310.1616377543157597No Hit
GTGGCTGGTGTGGCTAATGCCCTGGCCCACAAGTATCACTAAGCTCGCTT2927140.16162836739799777No Hit
GTTCCTGGCTTCTGTGAGCACCGTGCTGACCTCCAAATACCGTTAAGCTG2901960.1602379992259658No Hit
GACCCGGTCAACTTCAAGCTCCTAAGCCACTGCCTGCTGGTGACCCTGGC2898860.16006682601971883No Hit
GGCCCATCACTTTGGCAAAGAATTCACCCCACCAGTGCAGGCTGCCTATC2866240.15826564215269412No Hit
CTAAGCCACTGCCTGCTGGTGACCCTGGCCGCCCACCTCCCCGCCGAGTT2792950.15421877625403563No Hit
CTTCGGGTGGACCCGGTCAACTTCAAGCTCCTAAGCCACTGCCTGCTGGT2789010.15400122063061203No Hit
CTGGCTTCTGTGAGCACCGTGCTGACCTCCAAATACCGTTAAGCTGGAGC2778250.1534070839534451No Hit
CCGCCCACCTCCCCGCCGAGTTCACCCCTGCGGTGCACGCCTCCCTGGAC2722420.15032430972610022No Hit
CCCTGGACAAGTTCCTGGCTTCTGTGAGCACCGTGCTGACCTCCAAATAC2646030.14610627062119474No Hit
GCCTATCAGAAAGTGGTGGCTGGTGTGGCTAATGCCCTGGCCCACAAGTA2623180.14484455843966457No Hit
GTGTGCTGGCCCATCACTTTGGCAAAGAATTCACCCCACCAGTGCAGGCT2616120.1444547252667279No Hit
CTGGCCGCCCACCTCCCCGCCGAGTTCACCCCTGCGGTGCACGCCTCCCT2610560.14414771783874944No Hit
CCCACCAGTGCAGGCTGCCTATCAGAAAGTGGTGGCTGGTGTGGCTAATG2594960.14328633009118397No Hit
GCCTGCTGGTGACCCTGGCCGCCCACCTCCCCGCCGAGTTCACCCCTGCG2557180.14120022566150298No Hit
CGTGGATCCTGAGAACTTCAGGCTCCTGGGCAACGTGCTGGTCTGTGTGC2552510.1409423615088664No Hit
GTGACCCTGGCCGCCCACCTCCCCGCCGAGTTCACCCCTGCGGTGCACGC2525960.13947634582310597No Hit
CACGCCTCCCTGGACAAGTTCCTGGCTTCTGTGAGCACCGTGCTGACCTC2524400.13939020704834942No Hit
CACCCCACCAGTGCAGGCTGCCTATCAGAAAGTGGTGGCTGGTGTGGCTA2494630.13774639209674533No Hit
CCCCTGCGGTGCACGCCTCCCTGGACAAGTTCCTGGCTTCTGTGAGCACC2491280.13756141459967197No Hit
CCTGGCTTCTGTGAGCACCGTGCTGACCTCCAAATACCGTTAAGCTGGAG2479910.1369335954528887No Hit
CTATCAGAAAGTGGTGGCTGGTGTGGCTAATGCCCTGGCCCACAAGTATC2456500.13564096165990744No Hit
CCCTGGCCCACAAGTATCACTAAGCTCGCTTTCTTGCTGTCCAATTTCTA2441610.13481877809828888No Hit
GGTGACCCTGGCCGCCCACCTCCCCGCCGAGTTCACCCCTGCGGTGCACG2441160.1347939303748014No Hit
GCTGCCTATCAGAAAGTGGTGGCTGGTGTGGCTAATGCCCTGGCCCACAA2415960.13340245785950336No Hit
GCCGCCCACCTCCCCGCCGAGTTCACCCCTGCGGTGCACGCCTCCCTGGA2410840.1331197459833793No Hit
CTTCTGTGAGCACCGTGCTGACCTCCAAATACCGTTAAGCTGGAGCCTCG2386820.13179342972078173No Hit
CCCTGCGGTGCACGCCTCCCTGGACAAGTTCCTGGCTTCTGTGAGCACCG2353750.12996739813026956No Hit
TGAGAACTTCAGGCTCCTGGGCAACGTGCTGGTCTGTGTGCTGGCCCATC2350980.12981444658791336No Hit
GTCCGCCCTGAGCGACCTGCACGCGCACAAGCTTCGGGTGGACCCGGTCA2337700.12908116265921663No Hit
CCCACAAGTATCACTAAGCTCGCTTTCTTGCTGTCCAATTTCTATTAAAG2295240.126736641905266No Hit
CTGGCCCACAAGTATCACTAAGCTCGCTTTCTTGCTGTCCAATTTCTATT2257670.12466213307987918No Hit
GCCCTGGCCCACAAGTATCACTAAGCTCGCTTTCTTGCTGTCCAATTTCT2225800.1229023620853336No Hit
TTCACCCCTGCGGTGCACGCCTCCCTGGACAAGTTCCTGGCTTCTGTGAG2193200.121102282561575No Hit
CACCCCTGCGGTGCACGCCTCCCTGGACAAGTTCCTGGCTTCTGTGAGCA2189330.12088859213958279No Hit
CGAGTTCACCCCTGCGGTGCACGCCTCCCTGGACAAGTTCCTGGCTTCTG2186140.12071244938863833No Hit
GCAGGCTGCCTATCAGAAAGTGGTGGCTGGTGTGGCTAATGCCCTGGCCC2184480.12062078889755123No Hit
GCACGCCTCCCTGGACAAGTTCCTGGCTTCTGTGAGCACCGTGCTGACCT2174660.12007855635389143No Hit
CACAAGTATCACTAAGCTCGCTTTCTTGCTGTCCAATTTCTATTAAAGGT2144550.11841596756676348No Hit
CCACTGCCTGCTGGTGACCCTGGCCGCCCACCTCCCCGCCGAGTTCACCC2096050.11573793514644778No Hit
CTCCTAAGCCACTGCCTGCTGGTGACCCTGGCCGCCCACCTCCCCGCCGA2091310.11547620579237983No Hit
GTGACAAGCTGCACGTGGATCCTGAGAACTTCAGGCTCCTGGGCAACGTG2077430.11470979156569978No Hit
TGGGCAACGTGCTGGTCTGTGTGCTGGCCCATCACTTTGGCAAAGAATTC2074130.1145275749267917No Hit
AGAATTCACCCCACCAGTGCAGGCTGCCTATCAGAAAGTGGTGGCTGGTG2055540.11350108786094286No Hit
GGTGGACCCGGTCAACTTCAAGCTCCTAAGCCACTGCCTGCTGGTGACCC2050050.11319794563439578No Hit
CCATCACTTTGGCAAAGAATTCACCCCACCAGTGCAGGCTGCCTATCAGA2032040.11220348452326413No Hit
CTGTGTGCTGGCCCATCACTTTGGCAAAGAATTCACCCCACCAGTGCAGG2031610.11217974114304276No Hit
TCAACTTCAAGCTCCTAAGCCACTGCCTGCTGGTGACCCTGGCCGCCCAC2030400.11211292837544314No Hit
CAAGTTCCTGGCTTCTGTGAGCACCGTGCTGACCTCCAAATACCGTTAAG2027330.11194341168409533No Hit
CAAGCTCCTAAGCCACTGCCTGCTGGTGACCCTGGCCGCCCACCTCCCCG2024330.11177776019417889No Hit
GGACAAGTTCCTGGCTTCTGTGAGCACCGTGCTGACCTCCAAATACCGTT2003860.11064746486131574No Hit
GCTGGTCTGTGTGCTGGCCCATCACTTTGGCAAAGAATTCACCCCACCAG1991810.10998209804348473No Hit
CAACTTCAAGCTCCTAAGCCACTGCCTGCTGGTGACCCTGGCCGCCCACC1968010.10866792955681434No Hit
GAATTCACCCCACCAGTGCAGGCTGCCTATCAGAAAGTGGTGGCTGGTGT1952540.1078137200404786No Hit
GGTCAACTTCAAGCTCCTAAGCCACTGCCTGCTGGTGACCCTGGCCGCCC1931100.10662986405920913No Hit
CCTGGACAAGTTCCTGGCTTCTGTGAGCACCGTGCTGACCTCCAAATACC1928510.10648685160624795No Hit
GGCAAAGAATTCACCCCACCAGTGCAGGCTGCCTATCAGAAAGTGGTGGC1919860.10600922314365556No Hit
GGTGTGGCTAATGCCCTGGCCCACAAGTATCACTAAGCTCGCTTTCTTGC1915520.1057695806549098No Hit
CCTCCCTGGACAAGTTCCTGGCTTCTGTGAGCACCGTGCTGACCTCCAAA1909110.10541563863812169No Hit
TCTGTGTGCTGGCCCATCACTTTGGCAAAGAATTCACCCCACCAGTGCAG1905580.10522072205165334No Hit
AAGAATTCACCCCACCAGTGCAGGCTGCCTATCAGAAAGTGGTGGCTGGT1892240.10448412509315826No Hit
GGGCAACGTGCTGGTCTGTGTGCTGGCCCATCACTTTGGCAAAGAATTCA1857070.10254213745970457No Hit
GCCCACCTCCCCGCCGAGTTCACCCCTGCGGTGCACGCCTCCCTGGACAA1854830.10241845101390032No Hit
TGCTGGCCCATCACTTTGGCAAAGAATTCACCCCACCAGTGCAGGCTGCC1848020.10204242213179No Hit
TTCACCCCACCAGTGCAGGCTGCCTATCAGAAAGTGGTGGCTGGTGTGGC1845720.1019154226561874No Hit
CTCAAGGGCACCTTTGCCACACTGAGTGAGCTGCACTGTGACAAGCTGCA1837210.1014455245964578No Hit
CACCTCCCCGCCGAGTTCACCCCTGCGGTGCACGCCTCCCTGGACAAGTT1834710.10130748168819409No Hit
CGCCTCCCTGGACAAGTTCCTGGCTTCTGTGAGCACCGTGCTGACCTCCA1834210.10127987310654135No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)