FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005309931

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005309931
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15269033
Sequences flagged as poor quality0
Sequence length151
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTATA20200.028.354088145
GCCGTAA24100.014.139068145
GTAGATA97450.014.135518145
GGGGGGG10902000.010.645618145
CGCCGTT10004.2914326E-710.150046145
CGTGTAT23350.08.383336145
TAGATCA56350.08.362948145
TAGGGTT63350.08.354419145
AAGGGGG963400.08.007094145
AGGGGGG967800.07.6635494145
CGTCGTT28100.07.48224145
AAAGGGG1041900.07.4107738145
AAAAGGG1099700.07.027858145
AAAAAGG1292850.06.1741786145
GGTGTAT65900.05.8308306145
TCATTAA1748350.05.45717145
CATTAAA1707500.05.439117145
ATCATTA1773750.05.374936145
ATTAAAA1795050.05.3030796145
TCGGCCT204600.05.2773829
Produced by FastQC (version 0.11.5)