FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005309933

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005309933
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18291621
Sequences flagged as poor quality0
Sequence length151
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTATA22600.031.118868145
GTAGATA105400.014.514509145
CGCCGTT12300.013.557612145
AGTGTTA120650.011.838575145
GGGGGGG10092500.011.057496145
CGTGTAT37300.09.913383145
CTAACGG7350.00208182218.8708263
TAGATCA63250.08.711913145
TAAACGC18157.9489837E-108.3820743
GCCGTAA26750.08.131262145
CGTCGTT29850.08.015491145
AGGGGGG965750.07.43994145
AAGGGGG982300.07.3736386145
AAAGGGG1064150.07.2357273145
AAAAGGG1093450.06.829655145
CCTAACG11950.00305247336.6680012
AGCCGTT39650.06.5829387145
AAAAAGG1303700.06.0674696145
TCATTAA1741300.05.7002034145
CTAAACG26701.2641904E-65.6974292
Produced by FastQC (version 0.11.5)