FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005309942

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005309942
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences67029880
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACAGCTAGTGATCTCGTATG796800.11887235961037079TruSeq Adapter, Index 11 (97% over 37bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGAA390950.034.5269361
TCGGAAG406750.033.242082
CGGAAGA437350.031.041753
GATCGGA278450.021.017821
TATGCCG197150.019.94800645-49
CTCGTAT217800.018.0193440-44
CGTATGC220500.017.61856845-49
AGAGCAC809950.017.2624517
TCTCGTA214350.017.05084840-44
AGCACAC840150.016.71239
ATGCCGT242150.016.34276845-49
GAGCACA868200.016.1550548
GCCGTCT244650.015.91983750-54
GAAGAGC886900.015.7485425
ATCTCGT245650.014.95503240-44
CGTCTTC287050.013.9168850-54
CCGTCTT293450.013.52441550-54
CGTCTGA328650.011.94981215-19
TCGTATG239450.011.9019140-44
ACACGTC336200.011.7894710-14
Produced by FastQC (version 0.11.5)