FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005309947

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005309947
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences90037147
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGATTGCTCGTGTAGATCTC1035420.1149992013851794Illumina Single End PCR Primer 1 (96% over 31bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTCG621000.027.2521979
GAGCGTC717700.023.631298
AGAGCGT784250.021.8194967
ATCGGAA886100.020.0933381
TCGGAAG899100.019.9028952
AAGAGCG910150.019.7245776
CGGAAGA935900.019.1536543
CGCCGTA232000.016.87116455-59
TCGCCGT249800.015.39632255-59
GCCGTAT277650.014.30605355-59
GTCGCCG287900.013.81685455-59
TGGTCGC314050.012.7492350-54
CGTATCA313050.012.730947560-64
GAAGAGC1521400.012.2379615
GTGGTCG333450.012.12047650-54
CCGTATC302450.011.27396955-59
GGTCGCC331900.010.526690550-54
GCTCGTG413050.010.3610335-39
TCTCGGT419300.010.27578245-49
TGCTCGT413850.010.16940935-39
Produced by FastQC (version 0.11.5)