FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005309949

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005309949
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences57149809
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGATTGCTCGTGTAGATCTC720660.12610015896991011Illumina Single End PCR Primer 1 (96% over 31bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTCG419250.027.301469
GAGCGTC481500.023.8922928
TCTCGGT319950.022.8378728
CTCGGTG359050.022.7937539
AGAGCGT528250.021.8444947
GTCGATC39550.020.5371913
GCGTAGA69600.020.1455041
ATCGGAA575500.019.9477231
TCGGAAG589100.019.4238222
AAGAGCG604900.019.0799716
CGGAAGA615800.018.701673
TATCGGT48050.016.7458328
GTAGATC463850.015.744233
ATATCGG59100.015.453317
CGTAGAT80600.015.4038252
ATCGCGG36700.015.2076847
GATCTCG513800.015.0411616
CTCGGCG84350.014.9534359
TCTCGGG163550.014.8924438
ATCGGTG76150.014.3742079
Produced by FastQC (version 0.11.5)