FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005309979

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005309979
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences56762446
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTCG213400.027.5479359
GAGCGTC273000.021.6665128
AGAGCGT303350.019.5702697
ATCGGAA315650.019.2848031
TCGGAAG338200.017.8602942
CGGAAGA368450.016.38913
AAGAGCG375450.016.19826
CGCCGTA87700.014.58012255-59
TCGCCGT101200.012.40593655-59
GCCGTAT113850.011.54958655-59
GTCGCCG120150.010.93192455-59
TGGTCGC133550.010.00841250-54
CCGTATC133600.09.26709555-59
GTGGTCG146650.09.25277350-54
CGTATCA143200.09.18294860-64
GGTCGCC145500.08.6683150-54
GAAGAGC780850.08.2339885
TCTCGGT192500.07.591307645-49
ATCGGGA72500.07.23117971
TCGGTGG192950.07.10776645-49
Produced by FastQC (version 0.11.5)