FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005309989

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005309989
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15310075
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGATGTGTGGTGTAGATCTC317330.2072687429682742Illumina Single End PCR Primer 1 (96% over 31bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTCG134700.038.050089
GAGCGTC152100.033.9831858
AGAGCGT162500.031.8497497
ATCGGAA178000.028.9904841
TCGGAAG178300.028.955262
AAGAGCG184550.028.1209756
CGGAAGA186300.027.8220943
CGCCGTA67750.021.31496255-59
TCGCCGT73200.019.68837755-59
GCCGTAT74950.019.53818955-59
CGTATCA79000.018.3909660-64
GAAGAGC286200.018.335825
GTCGCCG80300.018.23645455-59
TGGTCGC85350.017.3943150-54
GTGGTCG89300.016.57620250-54
GATCGGA137300.016.084751
TCTCGGT99900.015.00633245-49
CTCGGTG106800.014.03682345-49
CGGTGGT110600.013.48872850-54
CCGTATC80000.013.17586155-59
Produced by FastQC (version 0.11.5)