FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005309990

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005309990
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13023685
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCACACATCATCTCGTATG321800.24708828568872787TruSeq Adapter, Index 7 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCACACATCATCTCGTAT140380.10778823351455444TruSeq Adapter, Index 7 (97% over 37bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGAA157700.030.857041
TCGGAAG159000.030.7843592
CGGAAGA162900.029.90373
AGAGCAC228800.021.3223847
TATGCCG68700.020.72361245-49
AGCACAC235200.020.6805429
GAGCACA238800.020.4598438
GAAGAGC248650.019.7659635
CTCGTAT72200.019.55896440-44
TCTCGTA70800.019.00372740-44
CGTATGC74150.018.80938745-49
ATGCCGT76000.018.7712145-49
GATCGGA122400.017.352471
ATCTCGT78600.017.0256340-44
GCCGTCT81200.016.9103850-54
CGTCTTC86800.016.2537150-54
AAGAGCA314050.015.8113346
CCGTCTT89900.015.62872350-54
CATCTCG87500.015.31045140-44
GGAAGAG350750.014.5082874
Produced by FastQC (version 0.11.5)