FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005310001

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005310001
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences75369972
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTATCGGTCGTGTAGATCTC1537090.20393930888019965Illumina Single End PCR Primer 1 (96% over 31bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTCG545450.043.093119
GAGCGTC627550.037.4786078
AGAGCGT682450.034.6767927
ATCGGAA737350.032.6158941
TCGGAAG756550.031.7416952
AAGAGCG774450.030.703526
CGGAAGA788100.030.4759353
CGCCGTA262800.022.12519655-59
TCGCCGT286300.020.30405655-59
GCCGTAT302600.019.42118855-59
GTCGCCG311000.018.85466855-59
GAAGAGC1293900.018.7639835
TGGTCGC332900.017.8677150-54
CGGTCGT327250.017.83663735-39
CGTATCA335800.017.3622160-64
GTGGTCG349450.017.08373650-54
ATCGGTC352750.016.70790530-34
TATCGGT365100.016.23407630-34
GTTATCG369700.015.93011730-34
TTATCGG376500.015.80028830-34
Produced by FastQC (version 0.11.5)