FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005310081

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005310081
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences128364040
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGTC633200.018.742399
GATCGGA689250.017.2982041
AGAGCGT721150.016.5269898
ATCGGAA738700.016.3138772
TCGGAAG783250.015.3644753
CGGAAGA867150.014.0198224
AAGAGCG894950.013.5359887
CGCCGTA276550.09.21756345-49
TCGCCGT293800.08.18776745-49
GCCGTAT325200.08.08384845-49
GTCGCCG336850.07.700991640-44
GAAGAGC1772850.07.376956
CCGTATC361950.07.31114145-49
CGTATCA370900.07.142539545-49
TGGTCGC371800.07.133082440-44
GGTCGCC399400.06.80716240-44
GTGGTCG403100.06.572016740-44
GCGTCGG117450.06.2350903145
TCTCGGT507950.05.51214135-39
TCGGTGG527550.05.288110335-39
Produced by FastQC (version 0.11.5)