FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005310125

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005310125
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences60540652
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTCG294000.013.1400619
GAGCGTC364300.010.7237618
CGCCGTA86850.010.56733755-59
AGAGCGT395350.010.0646697
ATCGGAA429500.09.5260411
TCGGAAG449250.09.089922
TCGCCGT106100.08.70473955-59
AAGAGCG488700.08.5722426
CGGAAGA484050.08.4169493
GCCGTAT117700.08.11784355-59
GTCGCCG123650.07.821019655-59
CGTATCA138250.07.02693160-64
TGGTCGC143500.06.860331550-54
GTGGTCG157100.06.312583450-54
GTCGAGT162650.06.088090435-39
CCGTATC139950.05.780866655-59
TTGTCGA179400.05.625317630-34
CTTGTCG196000.05.34123130-34
TCTCGGT207800.05.330560745-49
GAAGAGC879950.05.2549385
Produced by FastQC (version 0.11.5)