FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005310127

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005310127
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences96043990
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGTC570200.022.855689
GATCGGA618150.021.4543761
AGAGCGT635400.020.7385488
ATCGGAA651100.020.5050562
TCGGAAG689350.019.3989943
CGGAAGA742600.017.9985084
AAGAGCG763150.017.3619737
GAAGAGC1421450.09.8414486
CGCCGTA290550.09.31082145-49
TCGCCGT305000.08.73185545-49
GCCGTAT328300.08.390347545-49
CCGTATC353100.07.90780245-49
GTCGCCG345600.07.860535640-44
CGTATCA361750.07.70268445-49
TGGTCGC363050.07.69434340-44
GGTCGCC388150.07.17437540-44
GTGGTCG391150.07.17123540-44
GGAAGAG2247800.06.5556085
TCTCGGT482300.05.99633335-39
GCGTCGT446700.05.96787210-14
Produced by FastQC (version 0.11.5)