FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005310135

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005310135
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences53772927
Sequences flagged as poor quality0
Sequence length151
%GC42

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGAAGGAAGGTGTAGATCTC559290.10400958831941583Illumina Single End PCR Primer 1 (96% over 31bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTCG279700.032.6846929
GAGCGTC339250.027.011428
AGAGCGT380550.024.136567
ATCGGAA401350.023.046321
TCGGAAG419500.022.2273312
CGGAAGA457550.020.4710873
AAGAGCG469650.020.0517256
ATCGCGG41150.014.4462297
CGCCGTA133500.013.39153555-59
GCGTAGA73550.013.0917241
ATATCGG55700.012.88517957
TCGCCGT141650.012.49820555-59
GCCGTAT150500.012.11008855-59
GAAGAGC832600.011.6677025
CTCGGTG274700.010.8732759
GATATCG60850.010.7225696
CCGTATC167950.010.56695755-59
CGTATCA174400.010.44225960-64
GTCGCCG184150.010.34599455-59
AACTCGG119100.010.2260527
Produced by FastQC (version 0.11.5)