FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005310141

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005310141
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences80648480
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGATTACCGGTGTAGATCTC1124290.1394062231551047Illumina Single End PCR Primer 1 (96% over 31bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTCG340300.054.5711339
GAGCGTC416350.044.829568
AGAGCGT461650.040.681297
ATCGGAA470400.039.8135151
TCGGAAG503100.037.4023932
CGGAAGA538850.034.9616133
AAGAGCG547950.034.301816
CGCCGTA192100.020.17371655-59
TCGCCGT213750.018.164355-59
GAAGAGC1114500.017.4630345
GCCGTAT237100.016.52220555-59
GTCGCCG239350.016.45169355-59
TGGTCGC259900.015.25694650-54
TACCGGT259050.015.1001535-39
CGGTGTA265050.014.90054835-39
CCGTATC260250.014.50099155-59
CGTATCA271200.014.46691160-64
GTGGTCG281450.014.12481550-54
GGTCGCC272950.014.01757450-54
ACCGGTG286050.013.86239635-39
Produced by FastQC (version 0.11.5)