FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005310161

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005310161
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37926100
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTATGCACGAGTGTAGATCTC875600.23087003409261697Illumina Single End PCR Primer 1 (96% over 32bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTATGCACGAGTGTAGATCT771780.20349574567382359Illumina Single End PCR Primer 1 (96% over 33bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTCG284500.049.6531459
GATCGGA244850.048.5059131
GAGCGTC322550.044.020238
ATCGGAA356350.041.0686071
AGAGCGT347800.040.9488957
TCGGAAG364950.039.9681362
CGGAAGA392800.037.1103973
AAGAGCG397050.036.8913046
CGCCGTA209300.024.7767555-59
TCGCCGT216500.023.6715255-59
GTCGCCG227900.022.837355-59
GCCGTAT228050.022.7714255-59
GAAGAGC659250.022.4715235
TGGTCGC241950.021.52530550-54
GTGGTCG247600.021.1278150-54
CGTATCA246850.021.0142860-64
TGCACGA275000.019.26226430-34
TCTCGGT278150.019.22373845-49
CGAGTGT274650.019.20884335-39
ACGAGTG276950.018.9969735-39
Produced by FastQC (version 0.11.5)