FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005310167

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005310167
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences62440353
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAACGTCTGGTGTAGATCTC1641600.26290690573129843Illumina Single End PCR Primer 1 (96% over 32bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTCG569050.049.0395479
GAGCGTC638750.043.7682348
AGAGCGT678850.041.3856287
ATCGGAA747050.038.258931
TCGGAAG760800.037.5857662
AAGAGCG770100.037.055626
CGGAAGA794500.036.097053
GAAGAGC1208100.023.9510465
CGCCGTA283200.022.8740755-59
TCGCCGT300200.021.18270755-59
GCCGTAT312950.020.69958955-59
GTCGCCG329650.020.06437955-59
TGGTCGC344750.019.23057250-54
CGTATCA340450.018.84188560-64
GTGGTCG361350.018.48355350-54
GGAAGAG1728500.017.0591534
TAACGTC408000.016.74385630-34
TCTCGGT417450.016.62228445-49
GTAACGT415700.016.31513230-34
CCGTATC332950.016.16413555-59
Produced by FastQC (version 0.11.5)