FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005310260

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005310260
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences58409300
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA289500.018.1835171
ATCGGAA313050.016.8665032
TCGGAAG328400.016.1596363
CGGAAGA346500.015.3988214
TATGCCG116400.010.55029345-49
CTCGTAT135800.09.310348540-44
TCTCGTA133750.08.9760640-44
CGTATGC136350.08.91123940-44
CGATGTA136350.08.82615830-34
ATGCCGT147200.08.58900445-49
CCGATGT149100.08.41176630-34
TCGTATG154700.08.40719940-44
AGAGCAC668700.08.3693018
ACCGATG150100.08.27844730-34
TGCCGTC158950.08.07266345-49
CACCGAT156500.08.02328730-34
TATCTCG147250.07.818579735-39
GAGCACA728950.07.7968929
GCCGTCT164150.07.525455545-49
GAAGAGC755300.07.4576976
Produced by FastQC (version 0.11.5)