FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005310313

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005310313
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences95850009
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTACTAGGAGGTGTAGATCTC4016040.41899213593188084Illumina Single End PCR Primer 1 (96% over 32bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTCG940250.069.213289
GAGCGTC1044650.062.4550678
AGAGCGT1113250.058.7429547
ATCGGAA1149500.057.240151
TCGGAAG1177300.055.9275052
CGGAAGA1243800.052.9205133
AAGAGCG1253550.052.5079966
GAAGAGC1875500.035.3813745
GGAAGAG2652050.025.2782214
CGCCGTA601850.023.75294155-59
TCGCCGT630250.022.5422755-59
GCCGTAT646550.022.12196555-59
GTCGCCG680100.021.24598355-59
TGGTCGC706450.020.64389450-54
CGTATCA693150.020.60121360-64
GTGGTCG730600.020.08454350-54
CCGTATC682700.018.90542655-59
TCTCGGT813800.018.33395245-49
GGTCGCC727750.018.0773550-54
CTCGGTG870350.017.16271445-49
Produced by FastQC (version 0.11.5)