FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005310321

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005310321
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences47909410
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCTTCGACTGTGTAGATCTC557240.11631117978701888Illumina Single End PCR Primer 1 (96% over 31bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTCG305100.029.6234259
GAGCGTC362250.025.2298668
AGAGCGT390300.023.4720447
ATCGGAA435600.021.622721
TCGGAAG449850.021.0206452
AAGAGCG462750.020.4389176
CGGAAGA470750.020.1250113
CGCCGTA109550.017.38914155-59
TCGCCGT123800.015.11822255-59
GCCGTAT135050.014.245296555-59
GTCGCCG143450.013.55262355-59
TGGTCGC155650.012.62198250-54
CCGTATC150050.012.46375455-59
GAAGAGC778450.012.4479185
CGTATCA155000.012.41202960-64
GTGGTCG161200.012.214450-54
GGTCGCC166050.011.35120250-54
CTTCGAC186200.010.82960130-34
TCGACTG182150.010.62471130-34
CGACTGT194150.010.23010635-39
Produced by FastQC (version 0.11.5)