FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005310333

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005310333
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10661707
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTATA11100.037.227837145
ACGTCCG19050.026.639065145
AGGGGGG125950.025.038425145
AAGGGGG159750.022.781313145
CGCCGTT4752.746674E-1019.841095145
CGTCGTT12950.019.033781145
TGTACGG19150.016.278543145
AAAGGGG212000.015.935511145
GCCGTAA12750.015.920759145
AAAAGGG258300.014.875357145
GTAGATA85200.012.423077145
AAAAAGG391250.010.246763145
CGTATCA601600.09.411507145
TCATTAA579800.08.815092145
ATCATTA590800.08.601882145
AGAGCGG47550.08.385483145
CATTAAA576000.08.332042145
GTATCAT612450.08.226784145
CCGTATC633200.08.147271140-144
TATCATT639700.08.114328145
Produced by FastQC (version 0.11.5)