FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005310337

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005310337
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12464220
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTATA11450.037.98959145
GTACGTT16050.026.198221145
AGGGGGG158200.026.16667145
AAGGGGG198550.021.287148145
CGTCGTT13250.016.961515145
CGTGTAT23000.016.075377145
AAAGGGG255500.015.180347145
AAAAGGG295700.014.244382145
GTAGATA92150.013.138324145
GCCGTAA13400.011.90246145
CGCCGTT6857.2557086E-611.641821145
AAAAAGG425950.010.654536145
ATCATTA649650.08.849375145
GTATCAT687850.08.642491145
TCATTAA644750.08.511838145
CGTATCA665500.08.377166145
CATTAAA647050.08.257499145
CCGTATC701600.07.973007140-144
TATCATT705800.07.8474855145
AGAGCGG55100.07.762815145
Produced by FastQC (version 0.11.5)