FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005310341

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005310341
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14785247
Sequences flagged as poor quality0
Sequence length151
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGCCG25650.028.85063145
CCGTATA9800.014.806296145
CGCCGTT11401.6189006E-1011.455398145
GCGTCGG33350.010.007014145
AAGGGGG176400.09.994249145
CGTATCA328250.09.901837145
AGGGGGG177150.09.33762145
GTAGATA67050.09.197348145
ATCATTA337100.09.060785145
CCGTATC351350.08.713949145
AAAAGGG211000.08.492919145
GCCGTAT376600.08.160598140-144
TATCATT369500.08.128837145
GTATCAT369950.08.079727145
GTCGCCG457200.07.7597036145
TGGTCGC494650.07.6268964145
TCATTAA375000.07.4098606145
GGTCGCC489750.07.3476715145
AAAGGGG236800.07.261213145
CGCCGTA438900.07.055132140-144
Produced by FastQC (version 0.11.5)