FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005310342

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005310342
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16019055
Sequences flagged as poor quality0
Sequence length151
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGGGGG769250.012.158678145
CGCGTAG23550.08.9272913
AGGGGGG223400.08.405833145
GCGCTAG21554.1836756E-118.0734219
GCGTAGC26300.07.99377974
AAGGGGG236000.07.4962134145
CTTGAAA402600.07.3837028145
CCGTCTT529050.07.126409145
CCGCGTA30003.6379788E-127.00798942
AGAAATG233500.06.9243994145
AGCGCTA23451.5339538E-86.8010118
TATGCCG611300.06.7012863145
AAAAGGG267850.06.630996140-144
CGTCTTC518050.06.5908175140-144
GCCGTCT559550.06.5306406145
ATGCCGT601100.06.501389145
AAAGGGG299900.06.2616305145
TGCCGTC631300.06.2592874145
TAAACGC16302.9162594E-46.22635759
CTCGTAT697900.06.2229705145
Produced by FastQC (version 0.11.5)